Molecular detection and genotypic characterization of Toxoplasma gondii in wild waterfowls in Jilin Province, Northeastern China
作者单位：State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences Military Veterinary Institute Academy of Military Medical Sciences Key Laboratory of Jilin Province for Zoonosis Prevention and Control
会议名称：《中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工...》
会议日期：2016年
学科分类：090603[农学-临床兽医学] 09[农学] 0906[农学-兽医学]
基 金：Project support was provided,in part,by the National Natural Science Foundation of China(Grant Nos.31228022 and 31372430) the Special Fund for Agro-scientific Research in the Public Interest(Grant No.201303042) the National Science&Technology Pillar Program during the Twelfth Five-year Plan Period(Grant No.2013BAD12B04) the Open Funds of State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences(Grant No.SKLVEB2013KFKT006) the Science Fund for Creative Research Groups of Gansu Province(Grant No.1210RJIA006)
关 键 词：Toxoplasma gondii Wild waterfowls Genetic characterization Northeastern China
摘 要：Toxoplasma gondii can infect almost warm-blooded animals,including humans.Limited information about T.gondii infection in wild waterfowls is available in China.A total of249 wild waterfowls were sampled between April and July 2013 from Jilin Province,and the tissue samples were collected for the detection of T.gondii by a semi-nested PCR targeting the B1 gene.The overall prevalence of T.gondii in the wild waterfowls was 7.2%(18/249,95%confidence interval[CI]4.0-10.4),with the highest prevalence(22.0%,95%CI 10.5-33.5)in Anas formosa,followed by Anas platyrhynchos(20.0%,95%CI 6.0-44.0),Falcated teal(12.5%,95%CI 0.0-35.4),and Fulica atra(4.0%,95%CI 0.0-11.7).Of 18 positive samples,only 2 samples(Tg Wfjl1 and Tg Wfjl2)were genotyped completely,and one genotype,namely Toxo DB#9,was revealed.The result of this survey has implications for better understanding of the genetic diversity of T.gondii in China.This is the first report of prevalence and genotypic characterization of T.gondii in wild waterfowls in northeastern China.