Genomic Variability and Molecular Evolution of Asian Isolates of Sugarcane streak mosaic virus
会议名称：《中国植物病理学会2016年学术年会》
会议日期：2016年
学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治]
摘 要：Sugarcane streak mosaic virus(SCSMV),an economically important causal agent of mosaic disease of sugarcane,is a member of the newly created genus Poacevirus in the *** this study,we report the molecular characterization of three new SCSMV isolates from China(YN-YZ211 and HN-YZ49) and Myanmar(MYA-Formosa) and their genetic variation and phylogenetic relationship to SCSMV isolates from Asia and the type members of the family *** complete genome of each of the three isolates was determined to be 9 781 nucleotides(nt) in size,excluding the30 poly(A) *** analysis of the complete polyprotein amino acid(aa) sequences(3 130aa) revealed that all SCSMV isolates clustered into a phylogroupspecific to the genus Poacevirus and formed two distinct clades designated as group Ⅰ and group Ⅱ.Isolates YNYZ211,HN-YZ49 and MYAFormosa clustered into group Ⅰ,sharing 96.8%9.5%and 98.9%9.6%nt(at the complete genomic level) and aa(at the polyprotein level) identity,respectively,among themselves and 81.2%8.8%and 94.0%9.6%nt(at the complete genomic level) and aa(at the polyprotein level) identity,respectively,with the corresponding sequences of seven Asian SCSMV *** genetic analysis revealed greater between-group(0.190±0.004) than within-group(group Ⅰ=0.025±0.001 and group Ⅱ=0.071±0.003) evolutionary divergence values,further supporting the results of the phylogenetic *** analysis indicated that natural selection might have contributed to the evolution of isolates belonging to the two identified SCSMV clades,with infrequent genetic exchanges occurring between them *** findings provide a comprehensive analysis ofthe population genetic structure and driving forces for the evolution of SCSMV with implications for global exchange of sugarcane germplasm.