Halovulum dunhuangense gen.nov.sp.nov.,isolated from a saline terrestrial Spring
作者单位：State Key Laboratory Breeding Base of Marine Genetic Resources State Key Laboratory Breeding Base ofMarine Genetic Resources
会议名称：《福建省海洋学会2016年学术年会》
会议日期：2016年
学科分类：0710[理学-生物学] 07[理学] 071005[理学-微生物学]
关 键 词：Halovulum dunhuangense gen.nov.sp.nov. terrestrial Spring taxonomy water sand sediment mixed sample
摘 要：A bacterial strain,YYQ-30,isolated from a water sand sediment mixed sample collected from a terrestrial Spring located in Dunhuang,China,was characterized with respect to its morphology,physiology and taxonomy.The bacterium was found to be Gram stain-negative,aerobic,oxidase- and catalase-positive,non-flagellated,oval to rod-shaped(0.5-1 μm in width and 1.1-6.6 μm in length) and divided by binary fission.Growth was observed at 0-10.0%(w/v)NaCl with optimal growth at 0-3.0%.The pH and temperature ranges for growth are pH 6.0-9.0and 10-45℃(optimum growth at pH 7.0-8.5 and 30-37℃).The isolate could reduce nitrate to nitrite,hydrolyse aesculin and gelatine(weakly),but was unable to degrade Tween 80 or starch.Phylogenetic analysis based on 16 S rRNA gene sequences indicated that strain YYQ-30 belongs to the family Rhodobacteraceae and forms a distinct lineage with the type strain of Albimonas donghaensis and forms a branch within a cluster constituted by the type strains of the genera Albimonas,Rhodovulum,Albidovulum,Haematobacter,Tropicimonas;levels of 16 S rRNA gene sequence similarity between strain YYQ-30 and the members of related genera ranged from 94.1%to 89.7%.Strain YYQ-30 contains Q-10 as the predominant ubiquinone and summed feature 8(Cω7c/ω6c(70.0%),C(9.5%),summed feature 2(C 3OH/C iso Ⅰ and C aldehyde(6.9%),and Cω7c 11-methyl(6.0%) as the principal fatty acids.The polar lipids comprise diphosphatidylglycerol,phosphatidylethanolamine,phosphatidylglycerol,three unidentified phospholipids,two unidentifided aminolipids,and five unknown lipids.The pufLM gene was detected.The G+C content of the genomic DNA was determined to be 71.7 mol%.Based on the phylogenetic,chemotaxonomic and phenotypic data obtained in this study,strain YYQ-30 is considered to represent a novel species in a new genus within the family Rhodobacteraceae,for which the name Halovulum dunhuangense gen.***.sp.***.is proposed.The type strain of Halovulum dunhuangense is YYQ-30(= LMG 27418= MCCC 1A06483).