Pseudooceanicola atlanticus ***.,isolated from surface seawater of the Atlantic Ocean and reclassification of Oceanicola batsensis,Oceanicola marinus,Oceanicola nitratireducens,Oceanicola nanhaiensis,Oceanicola antarcticus and Oceanicola flagell
作者单位：State Key Laboratory Breeding Base of Marine Genetic Resources
会议名称：《福建省海洋学会2016年学术年会》
会议日期：2016年
学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 071005[理学-微生物学] 10[医学]
关 键 词：Pseudooceanicola atlanticus gen.nov.sp.nov. Atlantic Ocean taxonomy surface seawater reclassification
摘 要：A taxonomic study was carried out on strain 2211-Sllg,which was isolated from the surface seawater of the Atlantic *** bacterium was found to be Gram-negative,rod shaped without flagellum,oxidase positive and weakly catalase *** was observed at NaCl concentrations of 0.5-9%and at temperatures of 10-41℃.The isolate was incapable of gelatin hydrolysis and unable to reduce nitrate to nitrite,degrade aesculin and Tween *** the basis of16 S rRNA gene sequence similarity,strain 2211-S11g was found to be most closely related to Oceanicola batsensis HTCC2597(97.26%),followed by Oceanicola nitratireducens JLT1210(96.39%),whilst other species of genus Oceanicola shared 94.00-96.34%sequence ***,it showed low similarity to Oceanicola granulosus HTCC2516(94.79%),the type species of the genus *** analysis showed that strain 2211-S11 g formed a clade with six species currently classified in the genus Oceanicola,but strain *** HTCC2516 and strain *** M-M22 clustered with two other genera *** ANI values between strain 2211-S11g and two type strains(*** HTCC2597 and *** HTCC2516) are 91.86%and 91.81%*** digital DNA-DNA hybridization estimate values between strain 2211-S11g and two type strains(*** HTCC2597 and *** HTCC2516) are 23.4±2.4%and 20.0±2.3%,*** principal fatty acids were identified as summed feature 8(Cω7c/ω6c),C,Cω7c11-methyl and *** G+C content determined from the draft genome sequence is 64.1mol%.The respiratory quinone was determined to be Q-10(100%).Phosphatidylethanolamine,phosphatidylglycerol,an aminolipid,phosphatidylcholine,a phospholipid and three lipids were identified in the polar *** combined genotypic and phenotypic data also show that strain2211-S11g should not be assigned to the genus Oceanicola;consequently strain 2211-S11g is concluded to represent a novel species of a novel genus in the family Rhodobac