Dek35 encodes a PPR protein that affects cis-splicing of mitochondrial nad4 intron 1 and seed development in maize
作者单位：Shanghai Key Laboratory of Bio-Energy Crops School of Life Sciences Shanghai University National Maize Improvement Center of China China Agricultural University
会议名称：《第十届上海市植物生物学青年学术研讨会》
会议日期：2016年
学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种]
关 键 词：PPR splicing mitochondria NADH maize
摘 要：In higher plants, the splicing of organelle-encoded m RNA involves a complex collaboration with nuclear-encoded proteins. Pentatricopeptide repeat(PPR) proteins have been implicated in these RNA-protein interactions. In this study, we performed cloning and functional characterization of maize Defective kernel 35(Dek35), which encodes a new PPR protein. The dek35-ref mutant is a lethal seed mutant with developmental deficiency. Dek35 was cloned through Mutator tag isolation and was confirmed by four additional independent mutant alleles. Dek35 encodes a new P-type PPR protein that targets mitochondria. The dek35 mutation causes significant reduction of DEK35 proteins, and reduced splicing efficiency of mitochondrial nad4 intron 1. Mitochondrial complex analysis in dek35 immature seeds indicated severe deficiency of complex I assembly and NADH dehydrogenase activity. Transcriptome analysis of dek35 endosperm revealed enhanced expression in the alternative respiratory pathway and extensive differentially expressed genes related to mitochondrial function and activity. The results indicated that Dek35 is a new PPR protein that affects the cis-splicing of mitochondrial nad4 intron 1 and is required for mitochondrial function and seed development.