The Obtaining of shRNA sequences blocking the expression of nuclear factor kappa-B with high quality
作者单位：中山大学附属第二医院
会议名称：《第十五届全国泌尿外科学术会议》
会议日期：2008年
学科分类：1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100214[医学-肿瘤学] 10[医学]
关 键 词：nuclear factor kappa-B prostate carcinoma RNA interference
摘 要：【Objectives】To obtain shRNA sequences that can block the expression of nuclear factor kappa-B in the prostate cancer cell line Lncap with high quality. 【 Methods 】 According to nuclear factor kappa-B genetic information, we design siRNA1, siRNA2, siRNA3 and negative sequences, and those three siRNA sequences targeting the cds area of nuclear factor kappa-B gene and then form the corresponding four