Glucose deprivation-induced IL-6 expression and release isn’t mediated through NF-κB pathway in C2C12 myocytes
会议名称：《中国生理学会第23届全国会员代表大会暨生理学学术大会》
会议日期：2010年
学科分类：1001[医学-基础医学(可授医学、理学学位)] 10[医学]
基 金：supported by research grants from the National Natural Science Foundation of China(No.30570897)
摘 要：正Introduction:IL-6 is a pleiotropic cytokine secreted by many different *** muscle is one of important sources of IL-6,which might have relationship with energy *** the present research,we explore the effect of glucose deprivation（GD,glucose deprivation results in low intramuscular glycogen status） on skeletal muscle-derived IL-6 expression and release,and its signaling regulation by NF-kB pathway in C2C12 myocytes in ***:（1） C2C12 mouse myoblast was differentiated into myotube.（2） Differentiated C2C12 myotubes were replaced with DMEM medium containing 4.5 g/L glucose（glucose control,GC） or DMEM medium not containing glucose（glucose deprivation,GD） for 24 ***-6 mRNA level was analyzed by Real-Time ***-6 protein level in the medium was analyzed by ELISA.（3） 200 nmol/L NF-κB activation inhibitor was added into medium at the condition of glucose deprivation and glucose ***-6 protein in the medium was analyzed by ELISA after 24 h *** are expressed as mean±***:（1） As compared to glucose control, glucose deprivation was able to enhance IL-6 mRNA expression,and IL-6 mRNA expression was significantly increased by glucose deprivation at 24 h（0.87±0.36 vs 2.80±0.96,P0.05）.（2） As compared to glucose control, glucose deprivation was able to enhance protein release,IL-6 protein level in medium was significantly increased in glucose deprivation at 24 h（1285.38±118.62 vs 2617.11±389.20,P0.05）.（3） IL-6 protein level in glucose control + NF-κB activation inhibitor decreased compared with glucose control,but had no significantly effect（1285.38±118.62 vs 941.46±7.09,P0.05）.IL-6 protein level in glucose deprivation + NF-κB activation inhibitor decreased compared with glucose deprivation,but also had no significantly effect（2617.11±389.20 vs 2139.02±753.52,P0.05）. Conclusion:（1） Glucose deprivation was able to enhance skeletal muscle IL-6 mRNA expression.（2） Glucose deprivation was able to enhance skeletal muscle-derived