Study of low molecualr weight sulfated hyaluronic acid on the secretion of TNF-α、IL-1β from ALI/ARDS rats
会议名称：《中国药学会全国多糖类药物研究与应用研讨会》
会议日期：2008年
学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 100602[医学-中西医结合临床] 10[医学]
关 键 词：Low molecular weight sulfated hyaluronic acid Acute lung injury/acute respiratory distress syndrome Preparation Structure analysis Tumor necrosis factor-α Interleukin-1β mRNA
摘 要：正Objective Acute lunginjury （ALI） and acute respiratory distress syndrome （ARDS） represent inflammatory processes that are initiated by diverse systemic and/or pulmonary insults,resulting in a clinical syndrome of severe respiratory distress and refractory *** and ARDS are common,life-threatening causes of acute respiratory failure that arise from a variety of local and systemic *** some improvement in mortality with a lung-protective ventilator strategy,both morbidity and mortality remain high. According to the ALI and ARDS pathogenesis studies,anti-inflammatory and anti-coagulation therapies have become important treat *** is reported that glyeosaminoglycans （GAGs） has anti-inflammatory and anti-coagulation effect in recent *** molecular weight sulfated hyaluronie acid （LMWSHA） was selected as a new anti-acute lung injury material in our *** of LMWSHA on the sectretion of TNF-α、IL-1βfrom ALI/ARDS *** LMWSHA with different molecular weights （Mr） ranges were *** tested the effects of different compositions on TNF-αand IL-1βsecreted by lung alveolus macrophage,selected the most effective *** we studied its therapeutic effects on ARDS rats,and investigated its effects on mRNA transcription of TNF-α,IL-1βin the ARDS inflammatory *** The concentration of Rat TNF-αand Rat IL-βin the supernatant of cell culture was respectively 174.88±8.84 pg/mL and 148.55±3.30 pg/mL,obviously higher than the negative control group（15.50±2.98 pg/mL,21.22±1.65 pg/mL）（P0.05）.Among them compositions L-1-1,B-1-1 and B-2 were the most effective *** optical density values of group L-1-1,group B-1-1 and group B-2 were found to be lower than that of the model group,and the optical density value of group L-1-1 was found to be the lowest,demonstrating that three compositions of LMWSHA have inhibitive effect on the transcription of Rat lung alveolus macrophage TNF-α,RatIL-1βmRNA,with L-1-1 being the m