Purification and antifungal activities of an antimicrobial protein LJAMP2 expressed in Pichia pastoris

作     者：Shaohui Guo,Chengjian Xie,De Wang,Xiaowen Wang,Fei Kou,Xingyong Yang (Key Laboratory of Eco-Environments in Three Gorges Reservoir Region,Ministry of Education, School of Life Science,Southwest University,Chongqing 400716) 

会议名称：《重庆市生物化学与分子生物学学术会议》

会议日期：2009年

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：国家自然科学基金(No.30771388) 重庆市科委自然科学基金(No.2007BB1357) 

关 键 词：motherwort seed antimicrobial protein expression yeast 

摘      要：LJAMP2,a member of nonspecific lipid transfer protein,isolated from motherwort seeds,displays antimicrobial activity against *** LJAMP2 gene was cloned into the pPIC9K vector and transformed into Pichia pastoris using electrotransformation.A transformant with a high copy number of the gene integrated into the chromosome was obtained by YPD plates which contained 1.75 mg/mL G418.