Cryopreservation of Human L-02 hepatocytes Using Different Sugar as an Additive to the Cryoprotective Medium
会议名称：《第七届全国低温生物医学及器械学术大会》
会议日期：2010年
学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0836[工学-生物工程]
基 金：the funding support with Project No.50776060 from Natural Science Foundation of China Project No.07-0559
关 键 词：Sugars Cryoprotectant L-02 human hepatocytes
摘 要：This study describes the effect of glucose,sucrose or trehalose addition to a cryoprotective medium in order to reduce the MeSO concentration during cryopreservation of L-02 hepatocytes ***:L-02 human hepatocytes were cryopreserved with a cooling rate of 1℃/min down to-80℃and stored for two *** cryoprotectant solutions contained 2%or 5%MeSO(v/v) with glucose,sucrose or trehalose at a final concentration of 0.05,0.1,0.2 or 0.3 *** cell was processed and subjected to cryopreservation in solutions containing different concentrations of MeSO with different *** were thawed after two weeks,and then analysed by:the cell viability and 24h attachment was determined using the alamar blue assay,morphology activity of cells were ***:(1) glucose,sucrose, trehalose were cryopreserved L-02 hepatocytes which has a protective effect,The inclusion of trehalose as an additive to cryoprotective media for human hepatocytes enables reduction in the concentration of MeSO.(2) The one of the best concentration of formula which cryopreserved L-02human hepatocytes is 5%MeSO +0.3 M trehalose group.