Unravelling the biosynthetic mechanism of oosporein in Beauveriabassiana
作者单位：Key Laboratory of Insect Developmental and Evolutionary Biology
会议名称：《中国菌物学会第六届会员代表大会（2014年学术年会）暨贵州省食用菌产业发展高峰论坛》
会议日期：2014年
学科分类：07[理学] 09[农学] 0903[农学-农业资源与环境] 0713[理学-生态学]
关 键 词：Oosporein Beauveriabassiana polyketide synthase biosynthesis virulence,
摘 要：Insect pathogenic fiingi produce a variety of insecticidally andpharmaceutically active compounds,including the polyketideoosporein produced by the insect pathogenic fungi Beauveria *** other *** was first discoveredmore than fifty years ago,however,the genes responsible for its biosynthesiswere unknown until this *** onbioinformaticanalysisand targeted gene disruptions,we report the genecluster responsible for oosporein biosynthesis in the insect fungal *** synthase(PKS)OpS1 belongs to aniterative type Ⅰ PKS andis responsible for the biosynthesis of the precursor(orsellinic acid) for *** the hydroxylase OpS3 catalyzes the decarboxylation and hydroxylation of orsellinic *** laccase OpS4 then oxidizes the intermediate into a quinonoid compound,which is then dimerized through a radical *** biosynthesis of oosporein is enhanced by the transcription factor *** bioassays showed that oosporeinis important for the insect-killing process *** and may also function as an antagonist against other saprophytic *** resultof this study will facilitate the efficient production and development of oosporein as potential bioinsecticides or pharmaceuticals.