SH-groups contribute to the modulation of Ca2+-,Cd2+-and N-ethylmaleimide-induced mitochondrial swelling
作者单位：Department of Human and Animal PhysiologyIvan Franko National University ofLvivUkraine
会议名称：《2008北京国际生理科学学术大会》
会议日期：2008年
学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种]
关 键 词：mitochondrial swelling permeability transition state SH-groups
摘 要：Mitochondrial Ca overload results in the activation of permeability transition pore in mitochondrial inner membrane resulting in swelling,developing of low energy state and inhibition of oxidative phosphorylation of the *** latter could initiate cell damage and *** the mechanism involved in mitochondrial swelling are *** aim of this study was to investigate the effect of thiol-containing ligands reagent dithiothreitol (DTT) on the Ca-,Cd- and N-ethylmaleimide(NEM)-induced mitochondrial *** experiments were performed on isolated mitochondria obtained by differential *** swelling was measured *** swelling was analyzed by three parameters:i) initial slow phase(lag time) of response;ii) peak amplitude;iii) maximal rate of *** showed,that addition of DTT(1 mmol/L) to the mitochondrial suspension caused no significant effect on the Ca-induced mitochondrial *** rates of the process,stimulated by 15 and 100 mmol/L of Ca were equal to 0.198±0.038 and 1.297±0.082 A/minxmg protein,the lag time -22.1±3.8 and 11.3±0.1 s and the peak amplitude -0.292±0.007 and 0.295±0.010 A/mg protein *** of mitochondria with DTT during 1 min caused changes in the magnitudes of the analyzed *** rates of the process were equal to 0.189±0.037 and 1.245±0.133 A/minxmg protein [that comprise of(96±19)%and(96±10)%compare to control(without DTT)],the lag time -24.3±3.2 and 11.8±0.3 s[(110±14)%and(105±2)%compare to control(without DTT)],the peak amplitude -0.265±0.007 and 0.283±0.015 A/mg protein[(91±2)%and(96±5)%compare to control(without DTT)]).Interestingly,DTT caused an opposite effect on the Cd-induced mitochondrial swelling(15 and 50 mmol/L) and completely inhibits this *** also observed an increase of all above-mentioned parameters of the NEM-induced mitochondrial swelling after mitochondria incubation with *** particular,the rate of the NEM-st