Adenovirus-delivered angiopoietin-1 suppresses NF-κB and p38 MAPK and attenuates inflammatory responses in phosgene-induced acute lung injury
作者单位：Center of Emergency & Intensive Care UnitFudan University Medical Center of Chemical injuryFudan University Center LaboratoryJinshan HospitalFudan University
会议名称：《第十三次全国劳动卫生与职业病学术会议》
会议日期：2014年
学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
基 金：supported by National Youth Nature Science Foundation of China(No.81101412)to DKH a start-up fund of research fiom Jinshan Hospital(2012-2)to GX
关 键 词：Acute lung injury angiopoietin-1 cytokine NF-κB p38 MAPK phosgene
摘 要：Animals exposed to phosgene(Psg)result in acute lung injury(ALI).We have recently reported that angiopoietin-1(Ang1)reduces inflammation and vascular hyperpermeability in ALI *** this study,we examined whether the beneficial effects of adenovirus-delivered Angi(Ad/Ang1)on inflammatory responses in Psg-induced ALI rats are due to the suppression of the nuclear factor-kappa B(NF-κB)and p38 mitogen-activated protein kinase(MAPK)pathways,which play crucial roles in inflammatory responses in *** demonstrated that Psg increased Ang2 and inflammatory cytokines,such as tumor necrosis factor-a(TNF-a),interleukin(IL)-4(IL-4),IL-6,IL-8,and IL-10,in the serum and bronchoalveolar lavage fluid of ALI rats,determined by ***1 inhibits pro-inflammatory mediators(TNF-a,IL-6 and IL-8)and has no effect on anti-inflammatory mediators(IL-4 and IL-10).Furthermore,the inhibitory action of Ang1 was mediated by the suppression of the NF-κB and p38 MAPK pathways,leading to the attenuation of inflammatory responses of ***,Ad/Ang1 may provide a useful tool for the effective treatment in Psg-induced ALI.