Experimental research on the effect of RNA interference specific for Smad3 gene on the activated hepatic stellate cells
作者单位：Tianjin Second Peoples Hospital
会议名称：《中华医学会第十六次全国病毒性肝炎及肝病学术会议》
会议日期：2013年
学科分类：1001[医学-基础医学(可授医学、理学学位)] 100104[医学-病理学与病理生理学] 10[医学]
摘 要：Objective To construct an eukaryotic expression vector of RNA interference specific for Smad3,and to evaluate the effect of this siRNA on hepatic stellate cells in *** To design psilencer3.1-H1-hygro plasmid expressing short interfering RNAs(siRNA) that target Smad3 gene region by aid of computer designing on Ambion *** plasmid expressing small interfering RNA was transfected into the cultured cells via li-