The control of glial cell activity by dorsal root ganglion neurons
作者单位：School of Biomedical SciencesThe Chinese University of Hong Kong Faculty of DentistryThe University of Hong Kong Division of Life ScienceThe Hong Kong University of Science and Technology
会议名称：《中国药理学会第十一次全国学术会议》
会议日期：2011年
学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学]
基 金：The project supported by a grant from the Research Grants Council of the Hong Kong SAR(GRF476710)
摘 要：OBJECTIVE Pain sensations in the periphery are carried by primary sensory neurons whose cell bodies lay in the dorsal root ganglia(DRG).The present study focuses on examining the interactions between DRG neurons and glial cells in vitro in relation to their roles as detectors of tissue *** Acutely dissociated DRG cells were isolated from adult SD rats,and plated on poly-DL-ornithine and laminin-coated tissue culture *** in 3 cell groups were compared over time in culture: mixed DRG cells(45%neurons on plating),neuron-enriched cells(70%neurons on plating) and glial cells(99%glial cells on plating).Cell proliferation was determined by counting cells,protein assay and MTT assay.[H]cAMP production in the presence of specific receptor agonists and antagonists was assayed to detect Gs-coupled *** acidic fibrillary protein(GFAP),glutamine synthase(GS) and Toll-like receptor 4(TLR4) mRNA and protein expression were determined by real time PCR and immunocyto-chemistry, *** The presence of Gs-coupledβAR,CGRP,EP and IP receptors were identified in glial cell preparations although only CGRP receptors have been reported on satellite glial cells in ***,IP receptor-stimulated adenylyl cyclase activity in glial cells was significantly inhibited by the presence of DRG *** neurons did not overtly affect glial cell proliferation or the expression of GFAP(classical markers of glial cell activation),but appeared to inhibit the expression of TLR4 and prevent the loss of GS in glial *** Time-dependent changes occur in DRG glial cells in vitro with regard to the expression of TLR4,Gs-coupled receptors and glial cell markers,and all these changes are influenced by the presence of DRG neurons. Whether similar neuron-glial interactions occur in vivo remains to be determined.