Transcriptome-wide mapping of pea seed ageing reveals a pivotal role for genes related to oxidative stress and programmed cell death
作者单位：Germplasm Bank of Wild SpeciesKunming Institute of BotanyChinese Academy of Sciences Seed Conservation DepartmentRoyal Botanic Gardens Departamento de Fisiología VegetalCentro Hispano-Luso de Investigaciones Agrarias(CIALE)Facultad de Biología.Universidad de SalamancaC/Río Duero 1237185 SalamancaSpain
会议名称：《生态文明建设中的植物学：现在与未来——中国植物学会第十五届会员代表大会暨八十周年学术年会》
会议日期：2013年
学科分类：09[农学] 0902[农学-园艺学] 090202[农学-蔬菜学]
关 键 词：seed ageing programmed cell death glutathione transcriptome
摘 要：Understanding of seed ageing,which leads to viability loss during storage,is vital for ex situ plant conservation and agriculture *** the potential for regulation at the transcriptional level has not been fully ***,we studied the relationship between seed viability,gene expression and glutathione redox status during artificial ageing of pea(Pisum sativum) ***-wide analysis using microarrays was complemented with qRT-PCR analysis of selected genes and a multilevel analysis of the antioxidant *** degradation of DNA and RNA occurred from the onset of artificial ageing at 60% RH and 50℃,and transcriptome profiling showed that the expression of genes associated with programmed cell death,oxidative stress and protein ubiquitination were altered prior to any sign of viability *** 25 days of ageing viability started to decline in conjunction with progressively oxidising cellular conditions,as indicated by a shift of the glutathione redox state towards more positive values(-180 mV).The unravelling of the molecular basis of seed ageing revealed that transcriptome reprogramming is a key component of the ageing process,which influences the progression of programmed cell death and decline in antioxidant capacity that ultimately lead to seed viability loss.