Laboratory research of the first confirmed pregnant woman case of avian influenza A (H5N1) by etiology in China
会议名称：《华东六省一市生物化学与分子生物学学会2006年学术交流会》
会议日期：2006年
学科分类：1002[医学-临床医学] 100211[医学-妇产科学] 10[医学]
关 键 词：Avian influenza H5N1 subtype Laboratory study
摘 要：Objective To ascertain causation of a pregnant woman with undefined pneumonia reported from the People’s Hospital of Tongling city in Anhui province on November 2005. Methods The patient’s tracheal aspirates and serum being collected were tested by both RT-PCR and real-time PCR to detect viral nucleic acids of A/H5Nl,A/H7N7,A/H9N1,and were inoculated into special pathogen free(SPF) and were sequenced and analyzed. Results HA gene of A/H5N1 virus were positive in tracheal aspirates. The results of hemagglutinin(HA) gene of A/AnHui/1/2005 sequencing showed that the receptor specificity and the connecting peptide between HA1 and HA2 were still avian influenza origin. Conclusion The undefined pneumonia case was confirmed as the first human case of avian influenza (H5N1) virus infection by etiology in the mainland of china.