Genetic Environment and Promoter Sequences of the ESBL-producing Klebsiella pneumoniae Isolates from Patients with Lower Respiratory Infection in Multiple Medical Centers in China
作者单位：Department of Respiratory & Critical Care MedicinePeking University People’s Hospital Department of Respiratory & Critical Care MedicineGuizhou Province People’s Hospital Department of Respiratory MedicineAffiliated Union HospitalScience and Technology Wuhan Department of Respiratory Medicinethe First Affliated Hospital of Tsinghua University
会议名称：《中华医学会呼吸病学年会——2013（第十四次全国呼吸病学学术会议）》
会议日期：2013年
学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
摘 要：Objectives From Febrary 2010 to July 2011,158 of 416 Klebsiella pneumoniae(***) isolates producing extended-spectrum B-lactamase(ESBL)from patients with lower respiratory tract infection were collected from seven tertiary hospitals in *** study was conducted to analyse the genetic environment and promoter sequences of these 10 bla genes,18 bla genes and 2 bla genes in 158 ESBL-producing *** isolates. Methods The genetic environment was analysed by amplification and direct sequencing using various set of PCR primers. Results Sequence analysis revealed that the insertion sequence ISEcp1 was located upstream of the 5’ end of