Positional cloning and characterization reveal the molecular basis for soybean maturity locus E1 that regulates photoperiodic flowering
作者单位：Soybean Applied Genomics Research UnitNational Institute of Agrobiological Sciences Graduate School of AgricultureHokkaido University Faculty of AgricultureSaga University Biomolecular Research UnitNational Institute of Agrobiological Sciences
会议名称：《从植物科学到农业发展——2012全国植物生物学大会》
会议日期：2012年
关 键 词：flowering time maturity E1 photoperiodism positional cloning
摘 要：The maturity locus E1 has a large impact on flowering time in soybean,but the molecular basis for the El locus is largely *** positional cloning,we delimited the E1 locus to a 17.4-kb region containing an intron-free gene(E1).The E1 protein contains a putative bipartite nuclear localization signal and a region distantly related to B3 *** the recessive allele,a nonsynonymous substitution occurred in the putative nuclear localization signal,leading to the loss of localization specificity of the El protein and earlier flowering.E1 expression was significantly suppressed under short-day conditions and showed a bimodal diurnal pattern under long-day conditions,suggesting its response to photoperiod and its dominant effect induced by long day *** a functional E1 gene was transformed into the early-flowering cultivar Kariyutaka with low E1 expression, transgenic plants carrying exogenous E1 displayed late ***,the transcript abundance of E1 was negatively correlated with that of GmFT2a and GmFT5a, homologues of FLOWERING LOCUS T that promote *** molecular identification of the maturity locus E1 will contribute to our understanding of the molecular mechanisms by which a short-day plant regulates flowering time and maturity.