Comparison of two protein extraction methods for proteomic a...
Comparison of two protein extraction methods for proteomic analysis of chlorophyll-deficient mutants in Brassica Juncea L.
作 者：Rui Zhang~1,Haiheng Liu~2,Huixian Zhao~(1*),Shengwu Hu~(2*) 1 College of Life Sciences,Northwest A & F University,Yangling Shaanxi 712100,China 2 College of Agronomy,Northwest A & F University,Yangling Shaanxi 712100,China
会议名称：《第十一届全国植物基因组学大会》
会议日期：2010年
学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种]
摘 要：Total proteins were extracted from leaves of Brassica juncea *** five leaves period with two different protocols,TCA/acetone extraction method and polyethylene glycol fractionation method. Then the samples were analyzed by two-dimensional gel electrophoresis,and IPG gel strip pH and
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