Peptide-based Fluorescent Chemosensor for Cadmium Recognition
作者单位：Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu ProvinceState Key Laboratory of Applied Organic ChemistryCollege of Chemistry and Chemical EngineeringLanzhou University
会议名称：《中国化学会第九届全国无机化学学术会议》
会议日期：2015年
学科分类：081704[工学-应用化学] 07[理学] 080202[工学-机械电子工程] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学] 0802[工学-机械工程]
关 键 词：peptide fluorescent chemosensor cadmium ions
摘 要：As all we known,cadmium ions can cause serious diseases and even certain forms of cancers,and also environmental *** use of fluorescent chemosensors for Cd2+recognition could help to discover the cadmium carcinogen mechanism in vivo as well as to detect cadmium ion temporally in the environment.[1]Until now,a number of fluorescent chemosensors have been developed to monitor cadmium concentration,but most of them are organic dyes which may not be suit for in biological system study because of their toxicity and poor water solubility.[2]We propose that linking biological molecule and organic dyes together could reduce the cell toxicity and increase biocompatibility of the chemosensors.[3]A novel peptide fluorescent chemosensor（L）with a lysine backbone and both NH2 sites conjugated with cysteine and dansyl groups has been designed and synthesized by solid phase synthesis with Fmoc *** chemosensor is a promising analytical tool for detecting Cd2+based on chelation enhanced fluorescence（CHEF）effect by turn-on response in 100%aqueous *** designed,L exhibits excellent cell permeation and low biotoxicity as well as displaying high selectivity and *** chemosensor penetrated live He La cells and detected intracellular Cd2+by turn-on *** binding stoichiometry and affinity,interference test,p H sensitivity,fluorescence quantum yield,quantum mechanical calculations,and cytotoxicity of L to Cd2+were also ***,L exhibits excellent cell permeation and low biotoxicity with the limit of detection（LOD）for Cd2+about 52 n M,implying that L can be used as a highly selective and sensitive peptide fluorescent chemosensor in biological systems.