Preparation and Application of Anti-human MDR Related New Gene CA916798 Polyclonal Antibody

作     者：haijing wang,heping yang,yongfeng chen,xiangdong zhou Department of Respiration,Southwest Hospital,Third Military Medical University 

会议名称：《中华医学会呼吸病学年会——2011（第十二次全国呼吸病学学术会议）》

会议日期：2011年

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

摘      要：CA916798,a new gene,was cloned from multidrug-resistant(MDR) human lung adenocarcinoma cell line SPC-A-1/CDDP using suppression subtractive hybridization(SSH). Thiss tudy was to express and purify CA916798 fusion protein and to generate rabbit anti-human CA916798 polyclonal antibody,which was used to analyze CA916798 expression in tumor cells. Total RNA was isolated from A549 *** ORF of CA916798 was amplified by Touch down