Role of dorsal root ganglion P2X3 receptors in electroacupuncture analgesia
会议名称：《中国生理学会第23届全国会员代表大会暨生理学学术大会》
会议日期：2010年
学科分类：1005[医学-中医学] 100512[医学-针灸推拿学] 10[医学]
摘 要：Background:Extracellular ATP contributes to initiation and transmission of neuropathic pain at the spinal cord and dorsal root ganglion(DRG) sites via the P2X receptors and the P2X subtype plays an important role in this *** thousands years,electroacupuncture(EA) has been clinically used to manage and alleviate chronic ***,to date,the specific analgesic mechanisms in spinal and infra-spinal levels remain unclear. Aim:The aim of this study is to investigate the effect of EA on neuropathic pain produced by chronic construction injury(CCI) mediated by P2X receptor in DRG ***:In this study,the mechanical withdrawal threshold(MWT) and thermal withdrawal latency(TWL) were investigated,and the P2X receptor protein level and the expression location in DRG were evaluated by Western blotting and *** experiment was composed by 2 series,including 6 groups,i.e.,normal group,sham CCI group,CCI group,CCI+EA group,normal+EA group and CCI+non-acupuncture point EA group(CCI+NA-EA group):Series 1,Change of pain thresholds of rats after establishment of CCI *** measurement time points involved day 0(pre-operation), day 3,day 5,day 7,day 9,day 11,day 14,day 18 and day 21 after CCI *** treatment started at day 1 after CCI operation for uninterrupted 21 *** point wasZusanliof contralateral operated *** 2, change of P2X receptors expression in DRG was measured by immunohistochemical and western blotting experiments on day 14 after CCI ***:The pain thresholds were decreased while P2X receptor expression in DRG was up-regulated when neuropathic pain *** the CCI group and CCI+NA-EA group,the pain thresholds were decreased dramatically after operation compared with preoperation,of which,changes on day 14 were the most *** were no significant differences in the pain thresholds among normal group,sham CCI group and normal+EA group at any time point,and the P2X receptor expressio