Expression and Effect of Chemokine CXCL14 in Colorectal Carcinoma
作者单位：Medical Experimental Teaching CenterWenzhou Medical University Department of Obstetrics and GynecologyFirst Affiliated Hospital of Wenzhou Medical University Department of General SurgeryTaizhou First People′s Hospital Department of gastrointestinal surgerythe First Affiliated Hospital of Wenzhou Medical University Department of Microbiology and ImmunologyInstitute of Molecular Virology and ImmunologyWenzhou Medical University
会议名称：《2014浙江省妇产科学、围产医学学术年会暨妇产科常见疾病规范化治疗新进展、围产医学现状与进展专题学术论坛》
会议日期：2014年
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
关 键 词：Colorectal Carcinoma Chemokine CXCL14 lentivirus survival analysis
摘 要：Chemokine plays an important role in tumor proliferation and ***14,a member of CXCL chemokine family,shows various expressions in different tumors,even in the same tissue,.The expression of CXCL14 and its clinical significance in colorectal carcinoma are *** this study,the expressions of CXCL14 in colorectal carcinoma and their adjacent normal tissues were detected by quantitative PCR and immunohistochemistry ***-Meier survival curve and Cox regression model were applied to evaluate the clinical significance of the expression of CXCL14 in colorectal *** investigate the cellular effect,a replication-defective lentivirus overexpressed CXCL14 was constructed and transfected to HT29 colorectal carcinoma *** effect of CXCL14 on the proliferation of colorectal carcinoma cells and the change of cellular cycle distribution were investigated using a CCK-8 kit and flow cytometry,*** indicated that the expressions of CXCL14 mRNA and protein in colorectal carcinoma were at a remarkably reduced level when compared to those in normal tissues(P0.05).The clinical correlation analysis suggested that down-regulation of CXCL14 expression was related to lymph metastasis,tumor location,and clinicopathologic stage(P0.05).Kaplan-Meier survival analysis revealed that down-regulation of CXCL14 expression was correlated with poor prognosis(P0.01).Overexpression of CXCL14 by lentiviral transfection had an inhibitory effect on cell proliferation by arresting G1 cell *** data suggest that CXCL14 may play a role in the development and progression of colorectal carcinoma,and act directly as a potential cancer suppressor *** level of CXCL14 expression may be a valuable adjuvant parameter in predicting the prognosis of colorectal carcinoma and,thus,a potential therapeutic target.