Generation of conditional mutations using gene trap mutagenesis in zebrafish
作者单位：Department of Molecular Physiology & Biophysics Vanderbilt University Medical Center
会议名称：《2010·第一届斑马鱼学术研讨会》
会议日期：2010年
学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种]
摘 要：Conditional mutations allow gene function determination in a specific tissue or at a desired *** mutations are not yet available in *** fill this void,we have developed a gene trap cassette that can be inverted in succession by Cre and FIp,with one orientation being strongly mutagenic and the other being