Development of a monolithic polymer pipette for solid-phase extraction of liquiritigenin in rat plasma
作者单位：School of PharmacyGuangdong Pharmaceutical University
会议名称：《全国生物医药色谱及相关技术学术交流会（2012）》
会议日期：2012年
学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学]
关 键 词：Monolithic polymer pipette Solid phase extraction High performance liquid chromatography Liquiritigenin
摘 要：A monolithic polymer column with mixed-mode function was prepared by in situ polymerization in a 1000μL *** kinds of monomers,butyl methacrylate（BMA） and 2-（dimethylamino）ethyl methacrylate（DMAM）,were applied to constructing the mixed-mode function for the synthesized polymer *** solid-phase extraction properties for liquiritigenin（LQG） were evaluated by high performance liquid chromatography（HPLC） with a gradient elution *** extraction procedure was optimized in *** then,the maximum binding capacity and extraction recovery following the optimal extraction procedure were also *** curve was expressed as A=65.9C+4.53（r 2 =0.998） with a linear range of 0.151～1.80μg/*** limit of quantification（LOQ） was estimated as 0.015μg/ml and the limit of detection（LOD） as 0.0045μg/*** experimental results indicate that the monolithic polymer pipette presents good extraction efficiency for *** monolithic polymer envisages the potential for application to the enrichment of other flavonoids compounds being similar to the structure of LQG.