高渗氯化钠溶液复苏对失血性休克小肠黏膜细胞HO-1 mRNA表达及凋亡的影响(英文)
作者单位：浙江大学医学院附属第一医院急诊科 浙江大学医学院邵逸夫医院普外科
会议名称：《第七届全国创伤学术会议暨2009海峡两岸创伤医学论坛》
会议日期：2009年
学科分类：100218[医学-急诊医学] 1002[医学-临床医学] 10[医学]
基 金：Project(No.20061420) supported by the Education and Research Foundation of Zhejiang Province China
关 键 词：Hemorrhagic shock Resuscitation Sodium chloride solution Hypertonic saline Apoptosis Heme oxygenase-1 mRNA Intestinal mucosa Flow cytometry Reverse transcriptase polymerase chain reaction
摘 要：Objective:To investigate the early effects of hypertonic and isotonic saline solutions on Heme oxygenase-1(HO-1) mRNA expression and apoptosis of intestinal mucosa in rats with hemorrhagic shock. Methods:A model of rat with severe hemorrhagic shock was established in 21 Sprague-Dawley(SD) rats. The rats were randomly divided into the sham group,normal saline resuscitation(NS) group,and hypertonic saline resuscitation(HTS) group,with 7 in each group.We detected and compared the HO-1 mRNA expression and apoptosis in small intestinal mucosa of rats after hemorrhagic shock and resuscitation by SYBR Green I fluorescence quantitative reverse transcriptase polymerase chain reaction,FITC(fluorescein -iso-thiocyanate)-Annexin V/PI(propidium iodide) double staining method,and flow cytometry. Results:In the early stage of hemorrhagic shock and resuscitation,marked apoptosis of small intestinal mucosa in the rats of both NS and HTS groups was observed.The apoptotic rate of small intestinal mucosa in the NS group was significantly higher than that in the HTS group(P0.01).Among the three groups,HO-1 mRNA of mall intestinal mucosa in the HTS group had the highest level of expression;however,the differences were not statistically significant(P=0.612).There was a significant negative correlation between HO-1 mRNA expression and apoptosis of small intestinal mucosa in the rats of NS and HTS groups after hemorrhagic shock and resuscitation.Conclusion:In this rat model of severe hemorrhagic shock,the HTS resuscitation of small volume is more effective than the NS resuscitation in reducing apoptosis of intestinal mucosa.Meanwhile,HO-1 mRNA over-expression of intestinal mucosa may be one of the molecular mechanisms of HTS in the resuscitation of hemorrhagic shock.