Ghrelin protects MES23.5 cells against 1-Methyl-4-phenylpyridinium ioninduced cytotoxicity
会议名称：《中国神经科学学会第四次会员代表大会暨第八届全国学术会议》
会议日期：2009年
学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学]
关 键 词：oxidative stress Parkinson’s disease ghrelin MPP+
摘 要：Objective 1-Methyl-4-phenylpyridinium ion(MPP) has been widely used as a cellular model of Parkinson’s disease(PD),because it can elevate intracellular reactive oxygen species level and apoptotic death,eliciting a severe PD- like ***,a 28-amino acid peptide,is an endogenous ligand for the growth hormone secretagogue receptor (GHS-R).Our previous data showed that ghrelin could protect dopaminergic neurons against 1 -methyl-4-phenyl-1,2,3,6- tetrahydropyridine(MPTP)-induced neurotoxicity in *** the present study,MPP-treated MES23.5 cells which could express GHS-R were used to further explore the underlying molecular mechanisms of this protective *** The MDA level was measured by MDA Assay ***-PCR and western-blots were adopted to detect the changes of catalase, SOD1,caspase-3 protein expression,and Bax/Bcl-2 ***(1) Ghrelin could suppress MPPMnduced lipid peroxidation in MES23.5 cells.(2) Ghrelin promoted the expression of endogenous antioxidant defense components including CAT and SOD 1.(3) Ghrelin could attenuate MPP-induced up-regulation of Bax/Bcl-2 ratio and the activation of *** Our results suggest that the protective effects of ghrelin on MPP-induced cytotoxicity may be ascribed to its anti-oxidative properties,anti-apoptotic activity via inducing expression of SOD1 and catalase,and regulation of Bcl-2 and Bax expression.