The correlation between CB expression and the receptors of GDNF in the early development SN
会议名称：《中国神经科学学会第四次会员代表大会暨第八届全国学术会议》
会议日期：2009年
学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学]
关 键 词：glial cell line-derived neurotrophic factor GDNF dopaminergic neurons substantia nigra pars compacta tyrosine hydroxylase calbindin D28k Ret cell adhesion molecules
摘 要：正Many studies indicated that DA neurons containing calbindin D28k（CB） has ability of anti-degeneration,and GDNF can protect DA neurons from degeneration by promoting CB expression in DA *** purpose of this study was to investigate which transmembrane receptor of GDNF was involved in mediating the expressions of CB in SN during rat early development ***-PCR and Western blot were performed to detect CB expression in SN of perinatal SD rats at mRNA and protein level respectively;Western blot was also performed to detect the expression of the transmembrane receptors including Ret,neural cell adhesion molecule-140（NCAM-140）,intergrinβ1 and *** results showed: CB mRNA expression had no correlation with the above-mentioned four molecules;there was a positive correlation of there was a positive correlation of CB protein expression with both integrinβ1 and N-cadherin protein expression,while no correlation with Ret and NCAM-140 protein *** data suggested that the expression of CB mRNA and CB protein were regulated by different receptor combinations.