Study of high-level expression of Musca domestica cecropin with partner molecular in Escherichia coli
会议名称：《格莱姆抗菌肽——抗菌肽开发与应用技术研讨会》
会议日期：2009年
学科分类：08[工学] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种]
基 金：Supported by National Natural Science Foundation of China(No 30471504) Key Science Research Project of Guangdong Province(2005B33701004 )
关 键 词：Musca domestica Cecropin Partner molecular Fusion expression Ubiquitin
摘 要：Objective To utilize partner molecular for effective expressing Musca domestica cecropin,research that partner molecular influences expression of *** cecropin in ***. Methods Gene of mature cecropin and ubiquitin of *** were amplified by *** bioinformatics methods,we analyzed secondary structure of the fusion protein of thioredoxin-MC and thioredoxin-ubiquitin-MC’s *** expression plasmids of pET32a-MC and pET32a-Ubiquitin -MC were *** expression plasmids were transformed into *** and were induced with IPTG *** of protein expression of pET32a-MC and pET32a-Ubiquitin-MC by SDS-PAGE was finished Results The protein quantity of expression of pET32a-Ubiquitin&MC were significantly higher than that of *** Profile of mature peptide of cecropin was transformed by *** fusion protein of thioredoxin-MC isn’t toxic for host *** results showed the partner molecule ubiquitin can significantly result in high-level expression of peptide antibiotic of M. demostica cecropin.