The cell culture of forebrain neuron in zebrafish early stage embryos

作     者：Bin YANG,Gang PENG~* Institutes of Brain Science and State Key Laboratory of Medical Neuroscience,Fudan University,Shanghai 200032, China 

会议名称：《中国神经科学学会第九届全国学术会议暨第五次会员代表大会》

会议日期：2011年

学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学] 

关 键 词：Danio rerio forebrain neuron cell culture 

摘      要：正Objective The zebrafish（Danio rerio）,is an important model organism in nervous system development and animal behavior studies for its fast development and transparent embryos in early stage.Comparing to in vivo methods,in vitro cell culture investigation approach has advantages in achieving higher resolution morphological observation and identifying typesof neurites with immunohistochemistry.Methods We use zebrafish early stage embryos to investigatefunctions of signal molecules and receptors for nervous development.We have developed methods involving bleaching,digestion,dissociation,plating of forebrain neurons and followed by immunohistochemistry.Results The cell culture of forebrain neuron in zebrafish early stage embryos provides a new way of studying nervous development.