IFN-γ and CD8+ T cells Contribute to Hematopoietic System Dysregulation in p40(-/-)interleukin-2Rα(-/-) mice
会议名称：《第十届全国免疫学学术大会》
会议日期：2015年
学科分类：1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学]
关 键 词：HSC MHCII IFN γ CD8+ T
摘 要：Objective:We had found hematopoietic system dysregulation in p40-/-IL-2Rα-/-mice and wanted to explore the ***:leukocyte isolation,flow cytometry,bone marrow ***:Lineage-c-Kit+Sca-1+cells(LSK cells)were usually considered to contain hemopoietic stem and progenitor cells.p40-/-IL-2Rα-/-mice had increased number and percentage of bone marrow LSK cells compared with wild type ***,bone marrow LSK cells in p40-/-IL-2Rα-/-mice had high expression of MHCII molecules,indicating that these cells may act as antigen-presenting *** number and percent of erythrocytes,B cells,NK cells were reduced in the bone marrow of p40-/-IL-2Rα-/-mice,suggesting the hematopoiesis disorder of HSCs,while T cell infiltration were *** reconstitution ability of bone marrow cells from p40-/-IL-2Rα-/-mice was lower than control *** we generated IFN-γ-/-p40-/-IL-2Rα-/-mice and found these mice still contained liver inflammation but did not have a huge *** found T cell infiltration was decreased in the bone marrow of IFN-γ-/-p40-/-IL-2Rα-/-mice,and the percent and number of B cells and erythrocytes were ***,the percent,number and phenotype of bone marrow LSK cells in IFN-γ-/-p40-/-IL-2Rα-/-mice were completely recovered and close to wild type *** reconstitution ability of total bone marrow cells from IFN-γ-/-p40-/-IL-2Rα-/-mice was *** last,we generated CD8a-/-p40-/-IL-2Rα-/-mice which lacked CD8+T *** found CD8a-/-p40-/-IL-2Rα-/-mice did not develop liver inflammation with a normal percentage of B cells,NK cells and NKT *** in these mice,CD4+T cells had a less activated phenotype and reduced IFN-γ***,CD8a-/-p40-/-IL-2Rα-/-mice displayed a complete normal bone marrow hematopoietic ***:p40-/-IL-2Rα-/-mice had hemotopoietic system dysregulation mediated by IFN-γ.And in p40-/-IL-2Rα-/-mice,CD8+T cells were critical for liver inflammation and CD8+T cells derived IFN-γc