Biological characteristics of hEGF gene modified HaCaT cells and its preliminary application in tissue engineering skin model
会议名称：《中华医学会烧伤外科学分会2009年学术年会》
会议日期：2009年
学科分类：1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学]
摘 要：正Cell-based skin tissue engineering have been effectively applied toward the development of grafting templates for wound repair and regeneration,but remain limited by inadequate availability of a robust epidermal cell *** cells are spontaneously immortalized human epidermal cell,which maintain full epidermal differentiation capacity and display a high capacity for in vitro expansion. Epidermal growth factor（EGF） has been well known as an important promoter for wound repair and *** this study,we deliverd human EGF（hEGF） gene to HaCaT cells by stable gene transfection method and the biological characteristics of the genetically modified cells was *** assay detection showed that HaCaT cells modified with hEGF gene could produced more than 390 ng/L of EGF in the *** vitro wound-healing and MTT assay analysis indicated that the hEGF gene transfected cells had significant faster migration ability and little reduced proliferation ability compared with normal HaCaT *** and western-blot analysis revealed that differentiation markers K19 and integrin-β1 were elevated in hEGF gene modified HaCaT ***,tumorigenicity test showed that the genetically modified cells was *** constructed engineered skin equivalents by organotypic coculture of hEGF gene modified HaCaT cells,normal HaCaT cells and normal *** result indicated that the epidermal morphogenesis was markedly improved and more thick stratified epidermis was formed in skin equivalents constructed by genetically modified *** findings suggest that HaCaT cells modified with hEGF gene might be a promising seed cell for construction genetically modified skin equivalents which can secrete epidermal growth factor as a means to accelerate wound repair and regeneration. Furthermore,that can provided a research tool for investigation of such topics as epidermal organization and differentiation.