Molecular cloning and expression analysis of a Mpp6 gene from black tiger shrimp（Penaeus monodon）

作     者：MiaoMiao Sun,LiHua Qiu,ShiGui Jiang,FaLin Zhou,CaiYan Zhu,LiShi Yang,TianFeng Su,JianHua Huang (1.South China Sea Fisheries Research Institute,CAFS,Chinese Academy of Fishery Sciences,Guangzhou Guangdong 510300,China 2.College of Aqua-life Science and Technology,Shanghai Ocean University,Shanghai 201306,China) 

会议名称：《2011年中国水产学会学术年会》

会议日期：2011年

学科分类：0908[农学-水产] 09[农学] 

关 键 词：Molecular cloing Mpp6 mRNA expression Penaeus monodon 

摘      要：正In present study,a M-phase phosphoprotein 6（Mpp6） gene was obtained from the ovary and neurosecretory organ in eyestalk cDNA library of black tiger shrimp（Penaeus monodon）.By PCR testing,comparisons of nucleotides showed 95%homologies between the PCR product and the partial *** full length black tiger shrimp Mpp6（PmMpp6） cDNA have obtained,homology analysis of the deduced amino acid sequence of the PmMpp6 with other known Mpp6 sequences by MatGAT software revealed that the PmMpp6 was high homology with other invertebrates.A conserved signature sequence of the Mpp6 family was found in the PmMpp6 deduced amino acid *** of the tissue expression pattern of the PmMpp6 showed that the PmMpp6 mRNA was expressed in all tissues tested,with highest levels in lymphoid tissue and ***,the Mpp6 expression was found to be different in six stages of ovarian development.