Dynamic Changes of Apoptosis in DEF Cells induced by New Type Gosling Viral Enteritis Virus

作     者：Chen Shun Cheng Anchun Wang Mingshu Chen Xiaoyue (1.Avian Disease Research Center,College of Animal Science and Veterinary Medicine of Sichuan Agricultural University, 2.Key laboratory of Animal Disease and Human Health of Sichuan Province,Yaan,Sichuan,625014,China 3.Research Center of Animal Microecological Engineering,Sichuan Agricultural University,Yaan,Sichuan,625014,P.R.China) 

会议名称：《第四届第九次全国学术研讨会暨饲料和动物源食品安全战略论坛》

会议日期：2008年

学科分类：090603[农学-临床兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：Supported by National Natural Science Foundation of China(Grant No.39970561) the Major Program of the National Science and Technique(No.2004BA901A03) 

关 键 词：New Type Gosling Viral Enteritis Virus (NGVEV) Duck Embryo Fibroblasts(DEF) Apoptosis 

摘      要：The monolayer Duck Embryo Fibroblast (DEF) cells were experimentally infected with New Type Gosling Viral Enteritis Virus (NGVEV) and the dynamic changes of apoptosis were detected at different time points after NGVEV infection by using transmission electron microscopy (TEM),DNA agarose gel electrophoresis and Annexin V-FITC/PI stained Fluorescence-Activated Cell Sorter (FACS).The result shows NGVEV can induce infected cells undergo apoptosis and change regularly.A series of characteristic apoptotic morphological changes including shrink of the cells,chromatin condensation and margination,as well as formation of apoptotic bodies were observed by *** typical ladder pattern of DNA fragmentation was demonstrated by agarose gel *** using flow cytometry analysis of Annexin V-FITC/PI staining,the dead,viable,apoptotic and necrotic cells could be analysis quantitatively.