The essential role of dopaminergic system in the food-anticipatory activity in mice
会议名称：《中国神经科学学会第九届全国学术会议暨第五次会员代表大会》
会议日期：2011年
学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学]
关 键 词：antagonist dopamine D1 receptor D2 receptor food restriction histamine H1 receptor food-anticipatory activity
摘 要：Objective When food is available during a restricted and predictable time of the day,mammals exhibit food-anticipatory activity(FAA),a daily increase in locomotor activity preceding the presentation of *** many studies attempted to locate the food-entrainable circadian oscillator in the central nervous system,the mechanisms for FAA are a matter of *** present study was designed to clarify the role of dopaminergic and histaminergic systems in *** The mice were given access to food for 2 h(CT19-CT21) in ICR mice and for 4 h(CT 17-CT21) in gene-manipulated *** was monitored by infrared locomotor activity sensors and defined as the locomotor activity occurred 2 h before the availability of *** D,receptor(R),D2R,and histamine H,R-specific antagonists,and DR- and HR-knockout(KO) mice were used to clarify the role of dopamine and histamine receptors in FAA induced by food restriction(FR).In addition,brain monoamine concentrations on the 14 day of FR were determined by *** Pretreatment with DR antagonist SCH23390 at 1,3,or 10μg/kg significantly decreased FAA by 19%,26%or 19%,respectively,and DR antagonist raclopride at 22,67,or 200μg/kg significantly decreased FAA by 16%,36%or 41%,respectively,as compared with vehicle ***,co-administration of SCH23390 and raclopride synergistically inhibited FAA by 57%.Consistently,the levels of dopamine and its metabolites in the striatum and midbrain were significantly increased during FR-induced FAA,even in the mice pretreated with DR and DR ***,DR KO mice exhibited attenuated ***,pretreatment with pyrilamine at 2.5,5,or 10 mg/kg did not significantly decrease FAA,although it decreased the locomotor activity during the dark period in ad libitum mice,and H,R KO did not affect *** These results strongly indicate that dopaminergic system plays an essential role in the food-anticipatory activity in mice.