Cloning and Characterization of a High Light-Inducible Polypeptide from Porphyra yezoensis
会议名称：《中国海洋湖沼学会藻类学分会第七届会员大会暨第十四次学术...》
会议日期：2007年
学科分类：0710[理学-生物学] 07[理学] 0908[农学-水产] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种]
关 键 词：Porphyra yezoensis Ueda High light High light-inducible polypeptide(HLIP) Light-harvesting chlorophyll a/b-binding (LHC) proteins
摘 要：正Porphyra yezoensis Ueda grows intertidal zone and is one of the most important *** intertidal zone is one of the harshest environments on earth and the intertidal zone algae are frequently exposed to high temperature,high light and osmotic pressure between tidal up and *** the long evolution,P. yezoensis have evolved mechanisms for photo-acclimation that favor survival in high light（HL）.And high light-inducible polypeptide（HLIP）is one of the light resistant *** this paper,we reported the HLIP *** and expression analysis from *** analysis indicates that it contains an ORF of 387 bp without intron encoding a polypeptide of 128 amino acids with similarity to HliC from Synechocystis *** of *** has a molecular mass of 13 kDa and have a theoretical pI of *** PHDhtm program clearly predicted a membrane-spanning-helix between 54-83 sites which is the conservation motif of light-harvesting chlorophyll a/b-binding（LHC）***-PCR analysis shows the high light induces expression of HLIP and under low high light no *** addition,the lengtj of exposed to high light affected the expression of HLIP.