Pathologic Analysis of Glioblastoma Via Stereotactic Biopsies Combined with Enhanced MR Images and ~11C-methionine PET
作者单位：Departments of NeurosurgeryChonnam National University Research Institute of Medical Sciences Chonnam National University Hwasun Hospital & Medical SchoolGwangjuSouth Korea Departments of Brain Tumor Research LaboratoryChonnam National University Research Institute of Medical Sciences Departments of Biomedical EngineeringChonnam National University Research Institute of Medical Sciences Departments of PathologyChonnam National University Research Institute of Medical Sciences Departments of Nuclear MedicineChonnam National University Research Institute of Medical Sciences
会议名称：《中国抗癌协会神经肿瘤专业委员会第八届学术会议》
会议日期：2011年
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
关 键 词：biopsy 11C-methionine PET enhanced MRI glioblastoma LAT1 stereotaxic
摘 要：Objective:To obtain more representative biopsy specimens in glioblastoma,we combined MR stereotactic planning with 11C-methionine PET and analyzed the pathologic ***:From December 2009 to October 2010,we performed stereotactic biopsies in 12 patients with radiologically heterogeneous,ring-enhanced *** biopsied the MR enhanced lesions having higher 11C-methionine uptake and the MR non-enhanced lesions having lower uptake and analyzed differences in pathologic diagnoses between *** pathologic findings,including cell density,Ki-67 LI,microvessel density,and number of endothelial proliferations,were analyzed as correlating factors of the degree of 11C-methionine uptake（T/N ratio）.We performed immunohistochemical staining for L-amino acid transporter 1（LAT1） to demonstrate the correlation with T/N ratio and the pattern of ***:The final diagnosis of each specimen was *** T/N ratio correlated statistically with enhancement（p = 0.000）.The diagnostic failure rate was 33.3%（4/12 patients） when we selected only one MR enhanced,high 11C-methionine uptake lesion,and 40%（4/10 patients） when we selected one MR non-enhanced,low 11C-methionine uptake *** T/N ratio showed a statistical correlation with cell density depending on the degree of necrosis,LAT1 immunopositivity,microvessel density,number of endothelial proliferations,and Ki-67 LI（p = 0.002,0.032,0.057,0.24 and 0.741,respectively）.LAT1 was localized in the tumor cells,vascular endothelium,and the vicinity of endothelial ***:Multiple stereotactic biopsies combined with enhanced MR images and 11Cmethionine PET could provide the diagnostic yield in glioblastoma,which mostly differing cell densities depending on the degree of necrosis.