The histone deacetylase inhibitor promotes totipotency in the male gametophyte
作者单位：Plant Research InternationalBioscienceP.O.BOX 6196700 APWageningenThe Netherlands Max Planck Institute for Molecular GeneticsD-14195 BerlinGermany Laboratory of Molecular BiologyWageningen UniversityWageningenThe Netherlands
会议名称：《2013全国植物生物学大会》
会议日期：2013年
学科分类：09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种]
关 键 词：histone deacetylase histone deacetylases inhibitor male gametophyte embryogenic totipotency
摘 要：The haploid muhicellular male gametophyte of plants,the pollen grain,is a terminally differentiated structure whose function ends at *** pollen grains,the immature gametophyte retains its capacity for totipotent growth when cultured in *** embryo production from cultured immature male gametophytes is a widely used plant breeding and propagation technique that was described nearly 50 years ago,but one that is poorly understood at the mechanistic *** a chemical approach,we show that the switch to haploid embryogenesis is controlled by the activity of histone deacetylases(HDACs).Blocking HDAC activity with histone deacetylases inhibitor in cultured immature male gametophytes of Brassica napus leads to a large increase in the proportion of cells that switch from pollen to embryogenic *** growth is enhanced by,but not dependent on,the high temperature stress that is normally used to induce haploid embryogenesis in *** immature male gametophyte of Arabidopsis thaliana,which is recalcitrant for haploid embryo development in culture,also forms embryogenic cell clusters after histone deacetylases inhibitor *** deacetylases inhibitor treatment of immature male gametophytes for as little as eight hours was accompanied by hyperacetylation of histones H3 and H4,and by the upregulation of genes involved in cell-cycle progression,the auxin pathway and cell wall catabolism *** propose that the totipotency of the immature male gametophyte in planta is kept in check by an HDAC-dependent mechanism,and that high temperature or other stresses used to induce haploid embryo development in culture impinge on this HDAC-dependent pathway.