THE TRANSITION TEMPERATURE OF HEMOGLOBIN IS UNAFFECTED AT ELEVATED CALCIUM LEVELS IN RBC

作     者：Kelemen,Ch.,O. Stache and G.M. ArtmannUniversity of Applied Sciences,Aachen,Cellular Engineering,Ginsterweg 1,D-52428 Jul ich,Germany. E-Mail Address: artmann@fh-aachen.de 

会议名称：《2000上海科技论坛BIO-X研讨会暨中德医学生物物理和上海流变学研讨会》

会议日期：2000年

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学] 

摘      要：In the work presented here weanalyzed the temperaturc depcndence ofhuman red blood cell(RBC) deformation andRBC passage behavior in narrowmicropipettes (1.3-1.7μminner diameter).Asuddcn transition from blocking to passageappeared at a critical temperature of 36.4±1.6℃..In contrast,normal RBCs not loadedwith additional calcium exhibited atemperature transition at 36.4±0.3℃ [1].Viscosimetric measurements of highlyconcentrated hemoglobin solutions andenhanced calcium content showed a suddenviscosity drop between 35℃ and 37℃.Anenhanced intracellular calcium concentrationhindered the cell ***,an1.7fold increased aspiration force had to beapplied to observe an almost hundred percentpassage above 38℃.