Cloning and comparative study of trehalose-6-phosphate synthase genes in seaweed

作     者：Ge Zhao~1 Yanbin Feng~(1,2) Jinsong Xuan~1 Fuxin Wang~1 Lixian Qiao~(1,2) Wei Wang~1 Manli Weng~1 Delin Duan~(3**) Bin Wang~(1**) 1 Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101 2 College of Life Sciences,Qingdao Agricultural University,Qingdao 2 66109 3 Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071 

会议名称：《中国海洋湖沼学会藻类学分会第七届会员大会暨第十四次学术讨论会》

会议日期：2007年

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

摘      要：Trchalose is a non-reducing disaccharide of two glucose units that functions as a stress protection metabolite in the stabilization of biological structures under stress tolerance in various *** biosynthesis of trehalosc is catalyzed by a two-step process that involves trehalosc-6-phosphate synthasc(TPS)and trchalosc-6-phosphate phosphatase(TPP).In this study,the full-length cDNA sequence(3219 bp)of the trehalose-6-phosphate synthase gene of Porphyra yezoensis(PyTPS)was isolated by RACE(rapid amplification of eDNA ends)-PCR. PyTPS encodes the TPS protein of 908 amino acids with a calculated molecular mass of 101786 Daltons,consisting of a TPS domain at the N-terminus and a putative TPP domain at the *** putative TPS sequence was deposited in GenBank(NCBI)with the accession numbers AY729671(mRNA)and AAW27916(protein).The homology alignment for the TPS proteins from five different organisms indicates that the most closely related sequences to PyTPS were those from OsTPS and AtTPS,whereas the most distant sequence to PyTPS was the one from EcOtsAB.