LPA/LPA receptor 2 pathway protects LLC cells against the TNF-αinduced apoptosis
作者单位：College of life sciencesInner Mongolia University
会议名称：《生命的分子机器及其调控网络——2012年全国生物化学与分子生物学学术大会》
会议日期：2012年
学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种]
摘 要：Lysophosphatidic acid(LPA) is a bioactive lipid that influences cancer cell survival through activation of cell surface G protein-coupled ***,we provide the first evidence that LPA reduces the Lewis Lung Cancer(LLC) cell apoptosis induced by TNF-αthrough LPA receptor *** Human LPA receptor 2(hLPAR2) CDS was amplified from MKN28 cells’ total RNA by RTPCR and sub-cloned into pMD19-T ***-19T-hLPAR2 and expression vector pIRES2-EGFP were digested by with xho I