Microarray analysis to examine the gene expression changes in the brains of NR2B induced memory enhancement mice
会议名称：《中国神经科学学会第九届全国学术会议暨第五次会员代表大会》
会议日期：2011年
学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学]
关 键 词：NR2B transgenic mice gene expression profile RyR Homer-1c
摘 要：正Glutamate N-methyl-D-aspartate（NMDA） receptors play a pivotal role in different forms of *** dysfunction of NMDA receptors contributes to the pathology of CNS *** further investigate the underlying mechanisms of NMDA receptors in the brain processes,we analyzed the gene expression profiles of the hippocampus between NR2B overexpression induced memory enhancement mice（Tg mice） and the *** results revealed that 249 genes were significantly affected in Tg mice,which mainly involved in neurotransmission,signal transduction,cytoskeleton,hormone activity and ***,the intracellular calcium channel proteins, ryanodine receptor（RyR） 1 and 3,as well as functional related proteins such as histidine rich calcium binding protein （Hrc） and triadin 2（Trdn） were ***,our results showed that Homer-lc protein was also increased in Tg mice,and formed a complex with RyR protein in mice brain,suggesting that Homer-lc might be an important modulator on both intracellular calcium signaling and overall neuronal signaling by interacting with NMDA receptor and RyR *** blot and Real-time PCR results showed that the expression of phospho-CREB, c-fos,immediate early genes Egr2 and Egr4 were also up-regulated in Tg *** studies demonstrated that chronic increased activation of NMDA receptors modified the expression of a large number of genes,which may provide important clues for better understanding the molecular mechanism of NMDA receptor modulated learning and memory and CNS disorders.