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Nitric Oxide Enhances the Sensitivity of Alpaca Melanocytes ...

Nitric Oxide Enhances the Sensitivity of Alpaca Melanocytes to Respond to a-melanocyte stimulating hormone by Up-regulating Melanocortin-1 Receptor

作     者:DONG Yan-jun,CAO Jing,WANG Hai-dong,ZHANG Jie,ZHU Zhi-wei,BAI Rui,HAO Huan-qing,HE Xiao-yan,Fan Rui wen,DONG Chang-sheng~(**) College of Animal Science and Technology,Shanxi Agricultural University,030801,Taigu,Shanxi,China. 

会议名称:《中国畜牧兽医学会动物解剖学及组织胚胎学分会第五次会员代表大会暨第十六次学术研讨会》

会议日期:2010年

学科分类:0905[农学-畜牧学] 09[农学] 

基  金:sponsored by the China National Natural Science Foundation(Grant No.30571070 and 30671512) 

关 键 词:Nitric oxide(NO) a-melanocyte stimulating hormone(a-MSH) Melanocortin-1 receptor(MC1R) Alpaca Melanocyte 

摘      要:Nitric oxide(NO) and a-melanocyte stimulating hormone(a-MSH) have been correlated with the synthesis of *** NO-dependent signaling of cellular response to activate the hypothalamopituitary proopiomelanocortin system,thereby enhances the hypophysial secretion of a-MSH to stimulatea-MSH-receptor responsive *** this study we investigated whether an NO-induced pathway can enhance the ability of the melanocyte to respond to a-MSH on melanogenesis in alpaca skin melanocytes in *** is important for us to know how to enhance the coat color of *** set up three groups for experiments using the third passage number of alpaca melanocytes:the control cultures were allowed a total of 5 days growth;the UV group cultures like the control group but the melanocytes were then irradiated everyday(once) with 312 mJ/cm of UVB;the UV+L-NAME group is the same as group UV but has the addition of 300μM L-NAME(every 6 hours).To determine the inhibited effect of NO produce,NO produces were *** determine the effect of the NO to the key protein and gene of a-MSH pathway on melanogenesis,the key gene and protein of the a-MSH pathway were measured by quantitative real-time PCR and western *** results provide exciting new evidence that NO can enhance a-MSH pathway in alpaca skin melanocytes by elevated *** we suggest that the NO pathway may more rapidly cause the synthesis of melanin in alpaca skin under UV,which at that time elevates the expression of MC1R and stimulates the keratinocytes to secrete a-MSH to enhance the a-MSH pathway on melanogenesis. This process will be of considerable interest in future studies.

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