Molecular characterization of the OsSPO11-1 in rice
会议名称：《湖北省植物生理学会第十五次学术研讨会》
会议日期：2007年
学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 09[农学] 0901[农学-作物学]
关 键 词：Osspo11-1 RNAi meiosis polyploid rice
摘 要：The Spo11 protein of yeast has been found to be covalently bound to double-strand breaks (DSBs) during the early stages of meiosis,demonstrating a unique role of the protein in the formation of these *** of the SPO11 gene have been found in various eukaryotes,indicating that the machinery involved in meiotic recombination is conserved in *** take advantage of the sequence information of the SPO11-1,which already cloned in other plants to find out their homology in rice genome sequence database and full-length eDNA sequence *** we cloned the OsSPO11-1,which is constituted by fifteen exons,with 1146bp long CDS encode a 381 amino acid *** contains five conserved protein motifs that are homology to the family of SPO11 *** OsSPO11-1 gene over-express vector and RNAi vector were constructed in efforts to study its role in the meiosis process in both diploid rice and polyploid rice.