Retroviral Insertional Mutagenesis Screen for Genes Involved in Human Disease Modeling in Zebrafish
会议名称：《“基因、进化与生理功能多样性”海内外学术研讨会暨中国生理学会第七届比较生理学学术会议》
会议日期：2009年
学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种]
基 金：supported by NSFC (30721064 and 30730056) as well as 973 Program from MOST of P.R.China(2005CB52 2504 and 2006CB943801)
关 键 词：Zebrafish Insertional mutagenesis Retrovirus Cardiovascular development
摘 要：Through retrovirus insertion based mutagenesis screen in zebrafish（Danio reiro） we have obtained several insertions that reside in *** and WDR55 are two candidate genes we conducted further *** encodes a cytokine induced anti-apoptosis protein with a methyltransferase domain, whose ortholog in mice is called Anamorsin. Ciapinl/Anamorsin represents a novel antiapoptotic protein that shares no homology with Bcl-2 and Capase family *** mouse embryos has been reported to be smaller in size compared with wild type littermates, show defects in definitive hemato-poiesis in fetal livers,and begin to die between E12.5 and *** mount in situ hybridization in zebrafish embryos revealed a broad expression pattern of *** ciapinl embryos exhibits edema in pericardial ***55 is a member of WDR-containing protein *** studies have shown that PeBoW complex,which contains a component of WDR12,is required for matura- tion of 60S ribosomal *** is also reported that WDR55 is involved in rRNA processing and cell *** data showed that WDR55 zebrafish embryos begin to die between 7 days post fertilization（dpf） and 10 *** mount in situ hybridization with markers of hematopoietic stem cells c-myb and runxl showed decreased expression in WDR55 *** may imply a function of WDR55 in definitive *** study is interesting because ribosome deficiency has been implicated in several human diseases such as Diamond-Blackfan Anemia that has congenital erythroid *** zebrafish mutants might be useful for modeling and understanding pathogenesis of these human diseases.