Cloning,Sequencing and Analysis of Melatonin Receptor Subtype MT1 in ypothalamus-Pituitary-Gonadal Axis and Pineal of Female Bactrian Camel
作者单位：College of Veterinary MedicineGansu Agricultural University China Agriculture Vet.Bio.Science and Technology CO.LTD Lanzhou Department ofAgricultureDezhou University
会议名称：《首届（2012）中国骆驼大会暨中国畜牧业协会骆驼分会成立大会》
会议日期：2012年
基 金：supported by National Natural Science Foundation of China(No.30871904)
关 键 词：clone sequence MT1 Hypothalamus-Pituitary-Gonadal Axis Bactrian camel
摘 要：It is well known that melatonin is a coordinating signal for mammalian *** order to confirm the presence of melatonin receptors in hypothalamus-pituitary-gonadal axis and pineal of female Bactrian camel,we used a reverse transcriptase-polymerase chain reaction(RT-PCR) procedure to examine receptor MT1 *** length of MT1 gene was 452 ***-PCR assaying revealed the presence of the mt1(Mel1a) melatonin receptor subtype in reproduction axis and pineal which were obtained from the slaughterhouse in the Ningxia Autonomous Region,*** has been confirmed a high identity(above 85%)with melatonin receptor MT1 of other mammal known in *** with other tissue’s sequences,one base substitution changed the 108th TTC codon(encoding Phenylalanine) to TAC(Tyrosine) on *** there are base substitutions in pineal’s gene, encoded amino acid are coincident with pituitary and *** current results,the expression of MT1 receptor mRNA in brain and ovary,suggest that melatonin regulate reproduction function through not only neuroendocrine but also directly acting on the ovary in Bactrian camel