Zebrafish:a predictive model for assessing developmental toxicity and teratotoxicity of drugs
会议名称：《中国药理学会第十一次全国学术会议》
会议日期：2011年
学科分类：1001[医学-基础医学(可授医学、理学学位)] 10[医学]
关 键 词：zebrafish developmental toxicity teratotoxicity animal model
摘 要：OBJECTIVE Conventional assessment of developmental toxicity and teratotoxicity in mammalian models is laborious and time *** the early 1980s,alternative methods for assessing developmental toxicity have been developed,including in vitro cell differentiation assays using either primary cell cultures or immortalized cell lines,the in vitro rodent whole embryo culture test and the in vivo frog embryo teratogenesis assay(FETAX).In vitro tests have been of limited value in predicting the effect of drugs on human embryonic and fetal *** of the in vivo FETAX assay include that xenopus do not have a characterized chemical metabolic pathway and they are insensitive to halogenated aromatic *** screening technologies is required to generate data directly for risk assessment or for prioritization of compounds for further testing in *** is emerging as an alternative vertebrate animal model for assessing toxicity and safty of compounds,in part due to its transpatency,rapid development, and simplicity of compound *** Zebrafish of the AB wild-type strain were reared under standard *** were collected from natural spawnings,staged according to established *** known mammalian-teratogenic compounds were as follows:bisphenol A,ethanol,lead nitrate,lithium chloride,tobacco leachate,caffeine and sudanⅠ.Three mammalian non-tera-totoxicity compounds were sucrose,saccharin and *** were exposed to three concentrations(LC,MNLCand 1/2 MN-LC) in each compound from 6 hpf to 120 hpf,30 zebrafish wree treated with each *** treatment,10 zebrafish from each group were randomly selected and images were acquired using a dissecting *** assessed the endpoints of evelop-mental toxicity and teratotoxicity at 120 *** All 7 known mammalian-teratogenic compounds induced evelopmental toxicity in *** major teratotoxicity were pericardial edema,head mal