The Protection of Buthus martensi Karsch Venom Against Influenza Infection In MDCK Cells
会议名称：《第二届中国青年学者微生物遗传学学术研讨会》
会议日期：2006年
学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100401[医学-流行病与卫生统计学] 10[医学]
关 键 词：venom influenza ROS GSH SOD.
摘 要：Influenza virus infection induces an increase in free radical production in host cells. Experiments were performed to determine whether the Buthus martensi Karsch (BmK) scorpion venom inhibited influenza viral infection in cultures of Madin-Darby canine kidney (MDCK) cells. At the early hours of infection, we detected various intracellular biochemical parameters in influenza virus infected MDCK cells, focused on the level of radical oxygen specials (ROS), superoxide dismutase (SOD) and glutathione (GSH). It was found that the venom could remarkably decrease the viral hemagglutinin titer. Further results showed that the introduction of venom restrained the excessive generation of intracellular ROS aroused by influenza virus. Measurement of total GSH content suggested that venom could help cells maintain a more reduced state and elevate the tolerance of cells against oxidative stress. Consistent with the GSH levels, the venom appeared to increase the total SOD activity. Together, the data suggest that the venom may enhance antiviral response and protect against oxidative stress induced by influenza infection.