Development of a new UPLC-MS/MS method for the determination of tacrolimus and cyclosporine A in human whole blood
作者单位：The Laboratory of Clinical Pharmacy The People’s Hospital of Lishui Analytical and Testing Center of Wenzhou Medical University
会议名称：《2014浙江省医学会临床药学分会、浙江省中西医结合学会中药分会学术会议》
会议日期：2014年
学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学]
关 键 词：Tacrolimus Cyclosporine A Human whole blood Therapeutic drug monitoring UPLC-MS/MS
摘 要：An accurate and validated liquid chromatography method and a triple quadrupole mass spectrometry method were developed and validated for the determination of tacrolimus and cyclosporine A in human whole *** blood samples were prepared by precipitating protein with acetonitrile after adding Zn *** analytes were separated using a reversed-phase BEH C18 column(2.1*50 mm,1.7μm,Waters,USA)maintained at 60°*** mobile phase consisted of acetonitrile and water(both containing 10m M ammonium acetate by adding 0.1%formic acid)with a gradient elution pumped at a flow humane of 0.4 m L/*** analytes were detected with positive electrospray ionization in multiple reaction monitoring(MRM)mode for target fragment ions m/z 822.36→769.37 for tacrolimus,m/z 1220.95→1203.74 for cyclosporine A and m/z 285.1→193.1 for diazepam(IS).Good linearity was achieved to quantify the concentration ranges of0.5-10 ng/m L for tacrolimus and 10-500 ng/m L for cyclosporine A in human whole *** mean recoveries of tacrolimus and cyclosporine A from the whole blood exceeded 75.58%.The intra-run and inter-run assay precisions of tacrolimus and cyclosporine A were both less than 8.9%.The validated method proved stability of tacrolimus and cyclosporine A in human whole blood and the correlation study showed the capability of the method to be used as an alternative for whole blood analysis in therapeutic drug monitoring.